User talk:Joiejoie

Welcome to BugSigDB! We hope you will contribute much and well. You will probably want to read the help pages. Again, welcome and have fun! Svetlana up (talk) 06:13, 11 October 2024 (UTC)

Thank you for having me here. Joiejoie (talk) 11:21, 14 December 2024 (UTC)

hi joie

May i know why you have given the alpha diversity as increased. actually it is unchanged did u check the significance?

its p>0.05 Nithya (talk) 02:30, 5 May 2025 (UTC)

and also may i know why you have created 6 experiments?

may i know which are all figures included? Nithya (talk) 02:39, 5 May 2025 (UTC)

Hi @Nithya , You can change the alpha diversities to unchanged. This was just a draft, I am still annotating the paper for areas to curate and asking questions. I created six experiments for the various groups 0 and 1 that we will be using in the experiments. We have for Figure 2:

The participants were divided into four distinct groups for analysis:

1. Healthy-LB – Healthy individuals with low BMI (BMI < 24)
2. PCOS-LB – PCOS patients with low BMI (BMI < 24)
3. Healthy-HB – Healthy individuals with high BMI (BMI ≥ 24)
4. PCOS-HB – PCOS patients with high BMI (BMI ≥ 24)

🔸 Main types of group comparisons:

• PCOS vs. Healthy overall
• PCOS-LB vs. Healthy-LB
• PCOS-HB vs. Healthy-HB
• Healthy-LB vs. Healthy-HB (to assess BMI effect in healthy individuals)
• PCOS-LB vs. PCOS-HB (to assess BMI effect within PCOS patients)

Joy (talk) 09:48, 5 May 2025 (UTC)

ok joie. were u able to curate any figures today? Nithya (talk) 12:51, 5 May 2025 (UTC)

Yes, I'm on it. Joy (talk) 13:20, 5 May 2025 (UTC)

I have curated Fig S2 Nithya (talk) 02:10, 6 May 2025 (UTC)

Im Done with the curation. Experiment 5 and 6 is not required right? We curate bacteria alone right? or fungi too? Nithya (talk) 03:20, 6 May 2025 (UTC)

Hi @Nithya, I was having a tough one making the decision to curate fungi, I'll check with the slack community. I'll revert. Joy (talk) 05:50, 6 May 2025 (UTC)

HI @Joiejoie I have done a rough draft of the experiments and signature. i will curate the article in detail tomorrow. good day! Nithya (talk) 03:48, 6 May 2025 (UTC)

Well done @Nithya on your curation, I'll finish up and submit it for peer review on Slack. Joy (talk) 08:01, 7 May 2025 (UTC)

hi

its not over yet Nithya (talk) 12:49, 7 May 2025 (UTC)

we have to recheck all the experiments again there are many missing columns to be filled Nithya (talk) 12:49, 7 May 2025 (UTC)

Hi @Nithya, I have refilled them. Joy (talk) 12:54, 7 May 2025 (UTC)

oh ok i have not finished the supplementary yet Nithya (talk) 13:03, 7 May 2025 (UTC)

experiment 6 and 7 are yet to be finished. ill finish it by tonight. once im done ill send you a message. you can recheck all experiments and then we can send it to peer review tomorrow. ok Nithya (talk) 13:06, 7 May 2025 (UTC)

Ok, noted. I am working on experiment 6. Joy (talk) 13:08, 7 May 2025 (UTC)

We still need 3 more experiments for Fig. 3d to capture these groups:

• PCOS-LB vs. Healthy-LB (Experiment 8)
• Healthy-LB vs. Healthy-HB (to assess BMI effect in healthy individuals) (Experiment 9) and
• PCOS-LB vs. PCOS-HB (to assess BMI effect within PCOS patients) (Experiment 10) The statistical test is Kruskal–Wallis tests

Joy (talk) 13:22, 7 May 2025 (UTC)

do we need to report BMI?

we are checking relative abundance right? Nithya (talk) 01:36, 8 May 2025 (UTC)

hi joy

i dont think experiment 4 is reqd (healthy vs healthy)

between how did u mark the relative abundance from fig 2E? Nithya (talk) 01:50, 8 May 2025 (UTC)

Experiment 6 how you found from 2D? Nithya (talk) 01:54, 8 May 2025 (UTC)

we dont do comparison studies for healthy vs healthy. if you want you can confirm that in slack too. Nithya (talk) 01:56, 8 May 2025 (UTC)

there are many experiments to be deleted. Nithya (talk) 01:57, 8 May 2025 (UTC)

Experiment 1, 4 and experiment 11. these are for bacteria. LEFse analysis. I have Curated it so to avoid confusion i request you not to do further correction to it. If you feel anything to be added inform me before doing it because you have edited all the lefse to kruskal wallis. First kruskal wallis fig.2D cannot be curated. Nithya (talk) 03:22, 8 May 2025 (UTC)

before proceeding with kruskal wallis can u send this article and ask with the peers whether it can be curated. I strongly feel it cannot be curated but still u have curated i want to confirm. Nithya (talk) 03:31, 8 May 2025 (UTC)

Ok @Nithya, you can make the necessary adjustments. Joy (talk) 04:21, 8 May 2025 (UTC)

@Joiejoie, did u ask in slack whether fig 2E is curatable? Nithya (talk) 15:32, 8 May 2025 (UTC)

hi joy experiment 2,3,5,9,10 and 12 are not required. shall i ask mentor svetlana to delete these experiments? Nithya (talk) 20:18, 8 May 2025 (UTC)