Surgery-induced gut microbial dysbiosis promotes cognitive impairment via regulation of intestinal function and the metabolite palmitic amide/Experiment 15
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-3
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Cognitive disorder Cognition Disorders,Cognitive Defects,Cognitive Deficits,cognitive disease,cognitive disorder,organic mental disorder,Cognitive disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Aged and Aged+surgery+dexamethasone
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Aged+surgery
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Adult male C57BL/6 mice 18 months old that underwent surgery were grouped as Aged+surgery
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-3
Source: Supplementary Figure 5G
Description: Linear discriminant analysis Efect Size (LEfSe) was used to find species with significant differences at all taxonomic levels of the gut microbiota in the feces of Aged, Aged+surgery and Aged+surgery+dexamethasone groups of mice.
Abundance in Group 1: increased abundance in Aged+surgery
Revision editor(s): MyleeeA, Anne-mariesharp
