Surgery-induced gut microbial dysbiosis promotes cognitive impairment via regulation of intestinal function and the metabolite palmitic amide/Experiment 7
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to transplant Response to transplant,response to transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Aged+surgery+youngFecal Microbiota Transplantation mice
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Aged+surgery+agedFecal Microbiota Transplantation mice
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Adult male C57BL/6 mice 18 months old that underwent surgery and Fecal microbiota transplantation with aged samples were grouped as Aged+surgery+aFMT.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
Signature 1
Source: Figure 5K
Description: Linear discriminant analysis Efect Size (LEfSe) was used to find species with significant differences at all taxonomic levels of the gut microbiota in the feces of Aged+surgery+youngFMT and Aged+surgery+agedFMT groups of mice.
Abundance in Group 1: increased abundance in Aged+surgery+agedFecal Microbiota Transplantation mice
NCBI | Quality Control | Links |
---|---|---|
Cyanobacteriota | ||
uncultured rumen bacterium 4C0d-2 | ||
Epsilonproteobacteria | ||
rumen bacterium YS2 | ||
Campylobacterales | ||
Helicobacteraceae | ||
Helicobacter |
Revision editor(s): MyleeeA
Signature 2
Source: Figure 5K
Description: Linear discriminant analysis Efect Size (LEfSe) was used to find species with significant differences at all taxonomic levels of the gut microbiota in the feces of Aged+surgery+youngFMT and Aged+surgery+agedFMT groups of mice.
Abundance in Group 1: decreased abundance in Aged+surgery+agedFecal Microbiota Transplantation mice
Revision editor(s): MyleeeA, Anne-mariesharp