Surgery-induced gut microbial dysbiosis promotes cognitive impairment via regulation of intestinal function and the metabolite palmitic amide/Experiment 7

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-7-3

Curated date: 2025/04/30

Curator: MyleeeA

Revision editor(s): MyleeeA, Anne-mariesharp

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Aged+surgery+youngFecal Microbiota Transplantation mice
Group 1 name Corresponds to the case (exposed) group for case-control studies
Aged+surgery+agedFecal Microbiota Transplantation mice
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Adult male C57BL/6 mice 18 months old that underwent surgery and Fecal microbiota transplantation with aged samples were grouped as Aged+surgery+aFMT.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased
Simpson Estimator of species richness and species evenness: more weight on species evenness
decreased
Richness Number of species
decreased

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-7-3

Curated date: 2025/05/01

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Figure 5K

Description: Linear discriminant analysis Efect Size (LEfSe) was used to find species with significant differences at all taxonomic levels of the gut microbiota in the feces of Aged+surgery+youngFMT and Aged+surgery+agedFMT groups of mice.

Abundance in Group 1: increased abundance in Aged+surgery+agedFecal Microbiota Transplantation mice

NCBI Quality ControlLinks
Cyanobacteriota
uncultured rumen bacterium 4C0d-2
Epsilonproteobacteria
rumen bacterium YS2
Campylobacterales
Helicobacteraceae
Helicobacter

Revision editor(s): MyleeeA

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-7-3

Curated date: 2025/05/01

Curator: MyleeeA

Revision editor(s): MyleeeA, Anne-mariesharp

Source: Figure 5K

Description: Linear discriminant analysis Efect Size (LEfSe) was used to find species with significant differences at all taxonomic levels of the gut microbiota in the feces of Aged+surgery+youngFMT and Aged+surgery+agedFMT groups of mice.

Abundance in Group 1: decreased abundance in Aged+surgery+agedFecal Microbiota Transplantation mice

NCBI Quality ControlLinks
Akkermansia
Chitinophaga
Chitinophagaceae
Peptostreptococcaceae
Saprospirales
Saprospiria
Verrucomicrobiaceae
Verrucomicrobiales
Verrucomicrobiia
Verrucomicrobiota
Erysipelotrichaceae_ClostridiumErysipelotrichaceae_Clostridium

Revision editor(s): MyleeeA, Anne-mariesharp