Gut microbiota modulate CD8+ T cell immunity in gastric cancer through Butyrate/GPR109A/HOPX/Experiment 3

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Reviewed Marked as Reviewed by KateRasheed on 2025-7-29

Curated date: 2025/05/06

Curator: MyleeeA

Revision editor(s): MyleeeA

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Gastric juice Stomach secretion,Succus gastricus,Gastric juice,gastric juice

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Response to transplant Response to transplant,response to transplant

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Normal mouse (N)

Group 1 name Corresponds to the case (exposed) group for case-control studies

Gastric Cancer Mouse (Ca)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Gastric cancer mouse- 20 g C57BL/6J mi­ce at 6 weeks of age infec­ted with Helicobacter pylori and given N-methyl-N-nitrosourea (MNU) plus abnormal feeding cycles, to induce gastric cancer.

Group 0 sample size Number of subjects in the control (unexposed) group

4

Group 1 sample size Number of subjects in the case (exposed) group

5

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool

2

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-7-29

Curated date: 2025/05/06

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Supplementary Figure 4A

Description: Differential analysis of microbial community composition of gastric fluids between Gastric Cancer mouse and Normal mouse.

Abundance in Group 1: increased abundance in Gastric Cancer Mouse (Ca)

NCBI	Quality Control	Links
Alistipes
Anaerotruncus
Bacteria incertae sedis
Bacteroides
Clostridium
Colidextribacter
Helicobacter
Mucispirillum
Muribaculum
Oscillibacter
Parabacteroides
Tyzzerella
bacterium A2
unclassified Bacteroidota
unclassified Eubacteriales
unclassified Lachnospiraceae
unclassified Oscillospiraceae
Rikenellaceae RC9 gut groupRikenellaceae RC9 gut group
Clostridia vadinBB60 group UnclassifiedClostridia vadinBB60 group Unclassified
UnclassifiedUnclassified
TuzzerellaTuzzerella
Lachnospiraceae FCS020 groupLachnospiraceae FCS020 group
Family XIII UCG-001Family XIII UCG-001
uncultured Clostridium sp.

Revision editor(s): MyleeeA

Signature 2

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-7-29

Curated date: 2025/05/06

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Supplementary Figure 4A

Description: Differential analysis of microbial community composition of gastric fluids between Gastric Cancer mouse and Normal mouse.

Abundance in Group 1: decreased abundance in Gastric Cancer Mouse (Ca)

NCBI	Quality Control	Links
Dubosiella
Ligilactobacillus
Methyloversatilis
Pseudomonas
Vibrio

Revision editor(s): MyleeeA