The Role of Gut Microbiota in Male Erectile Dysfunction of Rats/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Diet (ND)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Long-term high-fat diet (HFD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The mice in the high-fat diet (HFD) groups were fed a HFD (carbohydrate, 20%; protein, 20%; fat, 60%; Research Diets). The treatment lasted for 24 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 2K
Description: Comparison of genus abundance between Normal Diet and High Fat Diet groups.
Abundance in Group 1: increased abundance in Long-term high-fat diet (HFD)
| NCBI | Quality Control | Links |
|---|---|---|
| Desulfovibrio |
Revision editor(s): MyleeeA
Signature 4
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 2 (H and J)
Description: Comparison of genus abundance between Normal Diet and High Fat Diet groups.
Abundance in Group 1: decreased abundance in Long-term high-fat diet (HFD)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Lactobacillus |
Revision editor(s): MyleeeA
