Comparative Analysis of the Gut Microbiota of Bat Species with Different Feeding Habits/Experiment 3

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Reviewed Marked as Reviewed by Svetlana up on 2025-2-11

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC, WikiWorks

Subjects

Location of subjects
Brazil
Host species Species from which microbiome was sampled. Contact us to have more species added.
Molossus molossus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Material entity in digestive tract Digestive tract contents,Ingested material entity,Material entity in digestive tract,material entity in digestive tract
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
DietDiet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of frugivorous, nectarivorous and hematophagous bats.
Group 1 name Corresponds to the case (exposed) group for case-control studies
Insectivorous
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Insectivorous adult male bats caught in southern Brazil. Insectivorous bats have a specialized diet based on arthropods, adaptations include echolocation calls that allow tracking and capturing small fast-moving prey.
Group 0 sample size Number of subjects in the control (unexposed) group
23
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.1
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-2-11

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC, WikiWorks

Source: "3.3 Microbial Composition" second paragraph text

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: increased abundance in Insectivorous

NCBI Quality ControlLinks
Atopobium
Candidatus Arthromitus
Fusobacterium
Ligilactobacillus
unclassified Actinomycetota
unclassified Bacilli
unclassified Erysipelotrichales
unclassified Lactobacillales
unclassified Pasteurellaceae
Chlamydia

Revision editor(s): YokoC, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-2-11

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC, WikiWorks

Source: "3.3 Microbial Composition" second paragraph text.

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: decreased abundance in Insectivorous

NCBI Quality ControlLinks
Actinomyces
Campylobacter
Clostridium
Edwardsiella
Helicobacter
Peptostreptococcaceae
Terrisporobacter
Ureaplasma
unclassified Acholeplasmataceae
unclassified Clostridiaceae
unclassified Enterobacteriaceae
unclassified Neisseriaceae

Revision editor(s): YokoC, WikiWorks