Smoking-related gut microbiota alteration is associated with obesity and obesity-related diseases: results from two cohorts with sibling comparison analyses/Experiment 5
Curated date: 2025/04/24
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, Anne-mariesharp
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking status measurement Smoking status measurement,smoking status measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Never smokers of the WELL-China cohort
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Current smokers of the WELL-China cohort
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Current smokers of the WELL-China cohort (the discovery cohort)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 1126
- Group 1 sample size Number of subjects in the case (exposed) group
- 1143
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- arcsine square-root
- Statistical test
- MaAsLin2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- Matched on: "sibling pair" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.sibling pair
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Curated date: 2025/04/24
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, Anne-mariesharp
Source: Fig. 1B (C1) & Table S2
Description: Smoking-related microbial genera identified in male participants of the WELL-China cohort.
Abundance in Group 1: increased abundance in Current smokers of the WELL-China cohort
Revision editor(s): PreciousChijioke, Anne-mariesharp
Signature 2
Curated date: 2025/04/27
Curator: PreciousChijioke
Revision editor(s): PreciousChijioke, Anne-mariesharp
Source: Fig. 1B (C1) & Table S2
Description: Smoking-related microbial genera identified in male participants of the WELL-China cohort.
Abundance in Group 1: decreased abundance in Current smokers of the WELL-China cohort
Revision editor(s): PreciousChijioke, Anne-mariesharp
