Characterization of the Tissue and Strain-Specific Microbiota of Anopheles funestus Giles (Diptera: Culicidae)/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Anopheles funestus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Midgut , Saliva-secreting gland Mesenteron,Midgut,midgut,Glandulae salivariae,Salivary gland,Saliva-secreting gland,saliva-secreting gland
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sampling site Sampling site,sampling site
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Anopheles funestus (Midgut)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Anopheles funestus (Salivary gland)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Adults Anopheles funestus that were three days old and had not been blood-fed were used for midgut dissections
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 25
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- N/A
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.01
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
Signature 1
Source: Figure 5a
Description: Differential abundance of genera in Anopheles funestus tissues between midgut (left) and salivary glands (right).
Abundance in Group 1: increased abundance in Anopheles funestus (Salivary gland)
| NCBI | Quality Control | Links |
|---|---|---|
| Pseudomonas | ||
| Klebsiella | ||
| Cedecea | ||
| Azospirillum | ||
| Agromyces |
Revision editor(s): MyleeeA
Signature 2
Source: Figure 5a
Description: Differential abundance of genera in Anopheles funestus tissues between midgut (left) and salivary glands (right).
Abundance in Group 1: decreased abundance in Anopheles funestus (Salivary gland)
| NCBI | Quality Control | Links |
|---|---|---|
| Acinetobacter | ||
| Cutibacterium | ||
| Flectobacillus | ||
| Aeromonas | ||
| Streptococcus |
Revision editor(s): MyleeeA
