Characterization of the Tissue and Strain-Specific Microbiota of Anopheles funestus Giles (Diptera: Culicidae)/Experiment 2
From BugSigDB
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Anopheles funestus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Midgut , Ovary Mesenteron,Midgut,midgut,Animal ovary,Female gonad,Female organism genitalia gonad,Female organism genitalia gonada,Female organism reproductive system gonad,Female organism reproductive system gonada,Female reproductive system gonad,Female reproductive system gonada,Genitalia of female organism gonad,Genitalia of female organism gonada,Gonad of female organism genitalia,Gonad of female organism reproductive system,Gonad of female reproductive system,Gonad of genitalia of female organism,Gonad of reproductive system of female organism,Gonada of female organism genitalia,Gonada of female organism reproductive system,Gonada of female reproductive system,Gonada of genitalia of female organism,Gonada of reproductive system of female organism,Ovaries,Ovarium,Ovum-producing ovary,Reproductive system of female organism gonad,Reproductive system of female organism gonada,Ovary,ovary
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sampling site Sampling site,sampling site
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Anopheles funestus (Midgut)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Anopheles funestus (Ovaries)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Anopheles funestus ovaries were dissected from female adults that were 15 days old and had had 3 blood meals.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 25
- Group 1 sample size Number of subjects in the case (exposed) group
- 25
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- N/A
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.01
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Source: Figure 5b
Description: Differential abundance of genera in Anopheles funestus tissues between midgut (left) and Ovaries (right).
Abundance in Group 1: increased abundance in Anopheles funestus (Ovaries)
| NCBI | Quality Control | Links |
|---|---|---|
| Klebsiella | ||
| Azospirillum | ||
| Agromyces | ||
| Aeromonas | ||
| Pseudomonas |
Revision editor(s): MyleeeA
Signature 2
Source: Figure 5b
Description: Differential abundance of genera in Anopheles funestus tissues between midgut (left) and Ovaries (right).
Abundance in Group 1: decreased abundance in Anopheles funestus (Ovaries)
| NCBI | Quality Control | Links |
|---|---|---|
| Klebsiella |
Revision editor(s): MyleeeA
