-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Claregrieve1, ChiomaBlessing, Victoria
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Bronchus , Nose Bronchi,Bronchial tissue,Bronchial trunk,Bronchus,bronchus,Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Atopic asthma allergic asthma,Atopic asthma,atopic asthma
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- bronchial brush specimens
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- nasal brush speciments
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- nasal brush specimen type
- Group 0 sample size Number of subjects in the control (unexposed) group
- 27
- Group 1 sample size Number of subjects in the case (exposed) group
- 27
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- decreased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Source: Figure 3
Description: Differential microbial abundance between paired bronchial brush (BB) and nasal brush (NB) specimen types
Abundance in Group 1: increased abundance in nasal brush speciments
NCBI | Quality Control | Links |
---|---|---|
Alloiococcus | ||
Corynebacterium | ||
Moraxella | ||
Staphylococcus |
Revision editor(s): Claregrieve1, WikiWorks
Signature 2
Source: Figure 3
Description: Differential microbial abundance between paired bronchial brush (BB) and nasal brush (NB) specimen types
Abundance in Group 1: decreased abundance in nasal brush speciments
NCBI | Quality Control | Links |
---|---|---|
Actinomyces | ||
Leptotrichia | ||
Porphyromonas | ||
Prevotella | ||
Streptococcus |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 2
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Claregrieve1, ChiomaBlessing, Victoria
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Bronchus , Mouth Bronchi,Bronchial tissue,Bronchial trunk,Bronchus,bronchus,Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- oral wash specimens
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- oral wash specimen type
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Figure 3
Description: Differential microbial abundance between paired bronchial brush (BB) and oral wash (OW) specimen types
Abundance in Group 1: increased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Actinomyces | ||
Leptotrichia | ||
Streptococcus |
Revision editor(s): Claregrieve1, WikiWorks
Signature 2
Source: Figure 3
Description: Differential microbial abundance between paired bronchial brush (BB) and oral wash (OW) specimen types
Abundance in Group 1: decreased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Corynebacterium | ||
Moraxella | ||
Prevotella | ||
Staphylococcus |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 3
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Claregrieve1, ChiomaBlessing, Victoria
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Sputum , Nose Expectoration,Sputum,sputum,Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- nasal brush specimens
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- induced sputum specimens
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- induced sputum specimen type
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Curated date: 2021/01/10
Curator: Lucy Mellor
Revision editor(s): Claregrieve1, WikiWorks, OdigiriGreat
Source: Figure 3
Description: Differential microbial abundance between paired Nasal brush (NB) and induced sputum (IS) specimen types
Abundance in Group 1: increased abundance in induced sputum specimens
NCBI | Quality Control | Links |
---|---|---|
Actinomyces | ||
Leptotrichia | ||
Porphyromonas | ||
Prevotella | ||
Streptococcus |
Revision editor(s): Claregrieve1, WikiWorks, OdigiriGreat
Signature 2
Curated date: 2021/01/10
Curator: Lucy Mellor
Revision editor(s): Claregrieve1, WikiWorks, OdigiriGreat
Source: Figure 3
Description: Differential microbial abundance between paired Nasal brush (NB) and induced sputum (IS) specimen types
Abundance in Group 1: decreased abundance in induced sputum specimens
NCBI | Quality Control | Links |
---|---|---|
Alloiococcus | ||
Corynebacterium | ||
Moraxella | ||
Staphylococcus |
Revision editor(s): Claregrieve1, WikiWorks, OdigiriGreat
Experiment 4
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Claregrieve1, ChiomaBlessing, OdigiriGreat, Victoria
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Nose , Mouth Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose,Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- oral wash specimens
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- oral wash specimen type
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Figure 3
Description: Differential microbial abundance between paired nasal brush (NB) and oral wash (OW) specimen types
Abundance in Group 1: increased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Actinomyces | ||
Leptotrichia | ||
Porphyromonas | ||
Prevotella | ||
Streptococcus |
Revision editor(s): Claregrieve1, WikiWorks
Signature 2
Source: Figure 3
Description: Differential microbial abundance between paired nasal brush (NB) and oral wash (OW) specimen types
Abundance in Group 1: decreased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Alloiococcus | ||
Corynebacterium | ||
Moraxella | ||
Staphylococcus |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 5
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Claregrieve1, ChiomaBlessing, Victoria
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Sputum , Mouth Expectoration,Sputum,sputum,Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- induced sputum specimens
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Figure 3
Description: Differential microbial abundance between paired induced sputum (IS) and oral wash (OW) specimen types
Abundance in Group 1: increased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Actinomyces |
Revision editor(s): Claregrieve1, WikiWorks
Signature 2
Source: Figure 3
Description: Differential microbial abundance between paired induced sputum (IS) and oral wash (OW) specimen types
Abundance in Group 1: decreased abundance in oral wash specimens
NCBI | Quality Control | Links |
---|---|---|
Corynebacterium | ||
Prevotella | ||
Staphylococcus |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 6
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Sputum , Bronchus Expectoration,Sputum,sputum,Bronchi,Bronchial tissue,Bronchial trunk,Bronchus,bronchus
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- bronchial brush specimens
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- induced sputum specimens
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- induced sputum specimen type
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Source: Figure 3
Description: Differential microbial abundance between paired bronchial brush (BB) and induced sputum (IS) specimen types
Abundance in Group 1: increased abundance in induced sputum specimens
NCBI | Quality Control | Links |
---|---|---|
Leptotrichia | ||
Streptococcus |
Revision editor(s): Claregrieve1, WikiWorks
Experiment 7
Curated date: 2021/01/10
Curator: WikiWorks
Revision editor(s): WikiWorks, Chloe, Claregrieve1, Victoria
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Nose Nasal sac,Nasus,Nose,Olfactory apparatus,Peripheral olfactory organ,Proboscis,nose
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- atopic asthma subjects
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- subjects with atopic asthma
- Group 0 sample size Number of subjects in the control (unexposed) group
- 11
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Zero-Inflated Negative Binomial Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Figure S6B
Description: Asthmatic subjects showed significant enrichment in a number of specific Staphylococcus taxa in NB samples compared to HCs.
Abundance in Group 1: increased abundance in atopic asthma subjects
NCBI | Quality Control | Links |
---|---|---|
Staphylococcus | ||
Neisseriaceae | ||
Corynebacterium | ||
Hydrogenophaga | ||
Staphylococcus epidermidis | ||
Planococcaceae |
Revision editor(s): Claregrieve1, WikiWorks, Chloe