Effects of amoxicillin treatment on the salivary microbiota in children with acute otitis media/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Lwaldron on 2023-10-20
Subjects
- Location of subjects
- Switzerland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Amoxicillin (2S,5R,6R)-6-,6-(p-hydroxy-alpha-aminophenylacetamido)penicillanic acid,6beta-[(2R)-2-amino-2-(4-hydroxyphenyl)acetamido]-2,2-dimethylpenam-3alpha-carboxylic acid,[H][C@]12SC(C)(C)[C@@H](N1C(=O)[C@H]2NC(=O)[C@H](N)c1ccc(O)cc1)C(O)=O,alpha-amino-p-hydroxybenzylpenicillin,Amolin,Amopenixin,amoxicilina,Amoxicillin anhydrous,amoxicilline,amoxicillinum,amoxycillin,AMPC,AX,C16H19N3O5S,Clamoxyl,InChIKey=LSQZJLSUYDQPKJ-VLWBPTPADT,Moxal,p-hydroxyampicillin,Amoxicillin,amoxicillin
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control(Unexposed to Amoxicillin)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Amoxicillin-exposed
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This is the group of children who were exposed to amoxicillin treatment for acute otitis media (AOM). They received amoxicillin as part of their treatment.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 18
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Chinelsy on 2023-11-6
Source: Supplementary file: Table 2.
Description: Changes in taxa abundance at different visits (A2 vs. A1)
Abundance in Group 1: increased abundance in Amoxicillin-exposed
| NCBI | Quality Control | Links |
|---|---|---|
| Burkholderiales | ||
| Fusobacteriaceae | ||
| Fusobacterium | ||
| Pseudomonadota |
Revision editor(s): Chinelsy
