Microbiome recovery in adult females with uncomplicated urinary tract infections in a randomised phase 2A trial of the novel antibiotic gepotidacin (GSK140944)
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Urinary tract infection INFECTION, URINARY TRACT,TRACT, INFECTION OF URINARY,urinary tract infection,urinary tract infection (disease),Urinary tract infection
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 1 (pre-dose)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 5 (end of dosing or post-dose)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This represented the condition at the end of the 5-day regimen of gepotidacin treatment in the GIT microbiome
- Group 0 sample size Number of subjects in the control (unexposed) group
- 22
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.01
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Within results text(under "Changes in detected abundances of bacterial genera"line 8-14), Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Day 5 (end of dosing or post-dose)
Signature 2
Source: Within results text(under "Changes in detected abundances of bacterial genera"line 8-14), Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: decreased abundance in Day 5 (end of dosing or post-dose)
| NCBI | Quality Control | Links |
|---|---|---|
| Dialister | ||
| Enterococcus | ||
| Fusobacterium | ||
| Parabacteroides | ||
| Prevotella sp. | ||
| Selenomonas | ||
| Treponema | ||
| Tyzzerella | ||
| Veillonella | ||
| unclassified Lachnospiraceae |
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Follow-up (visit around Day 28 ± 3 days).
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This represented the condition approximately one month post-treatment, after the cessation of gepotidacin in the GIT microbiome
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Within results text(under "Changes in detected abundances of bacterial genera"line 8-14), Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Follow-up (visit around Day 28 ± 3 days).
Signature 2
Source: Within results text(under "Changes in detected abundances of bacterial genera"line 8-14), Figure 3
Description: Relative abundances of assigned bacterial taxa
Abundance in Group 1: decreased abundance in Follow-up (visit around Day 28 ± 3 days).
Experiment 3
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 5 (end of dosing or post-dose)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This represented the condition at the end of the 5-day regimen of gepotidacin treatment in the pharyngeal cavity microbiome.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 2, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Day 5 (end of dosing or post-dose)
Signature 2
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 2, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: decreased abundance in Day 5 (end of dosing or post-dose)
Experiment 4
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Follow-up (visit around Day 28 ± 3 days).
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This represented the condition approximately one month post-treatment, after the cessation of gepotidacin in the pharyngeal cavity microbiome.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 2, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Follow-up (visit around Day 28 ± 3 days).
| NCBI | Quality Control | Links |
|---|---|---|
| Acidaminococcus | ||
| Faecalibacterium | ||
| Megasphaera | ||
| Peptoniphilus | ||
| Subdoligranulum | ||
| Veillonella | ||
| [Ruminococcus] torques | ||
| uncultured Oscillospiraceae bacterium |
Signature 2
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 2,, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: decreased abundance in Follow-up (visit around Day 28 ± 3 days).
Experiment 5
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 5 (end of dosing or post-dose)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This represented the condition at the end of the 5-day regimen of gepotidacin treatment in vaginal microbiome.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 3, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Day 5 (end of dosing or post-dose)
Signature 2
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 3, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: decreased abundance in Day 5 (end of dosing or post-dose)
Experiment 6
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Follow-up (visit around Day 28 ± 3 days).
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- this represented the condition approximately one month post-treatment, after the cessation of gepotidacin in vaginal microbiome
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 3, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: increased abundance in Follow-up (visit around Day 28 ± 3 days).
Signature 2
Source: within results text under "changes in detected abundances in bacterial genera", paragraph 3, Figure 3
Description: Relative abundances of assigned bacterial taxa.
Abundance in Group 1: decreased abundance in Follow-up (visit around Day 28 ± 3 days).
