Changes in gut microbiota composition and diversity associated with post-cholecystectomy diarrhea
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Li YD, Liu BN, Zhao SH, Zhou YL, Bai L, Liu EQ
Journal
World journal of gastroenterology
Year
2021
Keywords:
16S rRNA, Bifidobacterium, Cholecystectomy, Diarrhea, Microbiota, Post-cholecystectomy
BACKGROUND: Post-cholecystectomy diarrhea (PCD) frequently occurs in patients following gallbladder removal. PCD is part of the post-cholecystectomy (PC) syndrome, and is difficult to treat. After cholecystectomy, bile enters the duodenum directly, independent of the timing of meals. The interaction between the bile acids and the intestinal microbes is changed. Therefore, the occurrence of PCD may be related to the change in microbiota. However, little is known about the relationship between the gut microbiota and PCD. AIM: To better understand the role of the gut microbiota in PCD patients. METHODS: Fecal DNA was isolated. The diversity and profiles of the gut microbiota were analyzed by performing high-throughput 16S rRNA gene sequencing. The gut microbiota were characterized in a healthy control (HC) group and a PC group. Subsequently, the PC group was further divided into a PCD group and a post-cholecystectomy non-diarrhea group (PCND) according to the patients' clinical symptoms. The composition, diversity and richness of microbial communities were determined and compared. RESULTS: In the PC and HC groups, 720 operational taxonomic units (OTUs) were identified. The PC group had fewer OTUs than the HC group. β-diversity was decreased in the PC group. This indicated decreased microbial diversity in the PC group. Fifteen taxa with differential abundance between the HC and PC groups were identified. In the PCD group compared to the PCND group, significant decreases in microbial diversity, Firmicutes/Bacteroidetes ratio, and richness of probiotic microbiota (Bifidobacterium and Lactococcus), and an increase in detrimental microbiota (Prevotella and Sutterella) were observed. Moreover, a negative correlation was found between Prevotella and Bifidobacterium. Using a Kyoto Encyclopedia of Genes and Genomes functional analysis, it was found that the abundances of gut microbiota involved in lipid metabolism pathways were markedly lower in the PCD group compared to the PCND group. CONCLUSION: This study demonstrated that gut dysbiosis may play a critical role in PCD, which provides new insights into therapeutic options for PCD patients.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-27
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Postcholecystectomy syndrome Postcholecystectomy Syndrome,postcholecystectomy syndrome,Postcholecystectomy syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy control (HC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- post-cholecystectomy (PC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who have post-cholecystectomy (PC) syndrome are difficult to treat.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 31
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-27
Source: Fig. 1D
Description: Comparison of gut microbiota structure and abundance between post-cholecystectomy (PC) and healthy control (HC) groups. Logarithmic linear discriminant analysis scores identify the taxa with the greatest differences in abundance between the healthy control and post-cholecystectomy groups.
Abundance in Group 1: increased abundance in post-cholecystectomy (PC)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacteriaceae | ||
| Bifidobacteriales | ||
| Actinomycetota | ||
| Clostridiaceae 1Clostridiaceae 1 | ||
| Veillonellaceae | ||
| Selenomonadales | ||
| Negativicutes |
Revision editor(s): Chinelsy
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-26
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diarrhea Diarrhea,Diarrhoea,Watery stool,diarrhea
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- post-cholecystectomy non-diarrhea (PCND)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- post-cholecystectomy diarrhea (PCD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group included patients who experienced post-cholecystectomy diarrhea (PCD). These individuals had diarrhea as a clinical symptom following gallbladder removal.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-26
Source: Fig.2C
Description: Comparison of gut microbiota structure and abundance between the post-cholecystectomy non-diarrhea (PCND) and post-cholecystectomy diarrhea (PCD) groups. Linear discriminant analysis and effect size analysis show the taxa with the greatest differences in abundance between the PCND and PCD groups. Yellow bars: PCND group-enriched taxa; Green bars: PCD group-enriched taxa.
Abundance in Group 1: decreased abundance in post-cholecystectomy diarrhea (PCD)
Revision editor(s): Chinelsy, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-26
Source: Fig.2C
Description: Comparison of gut microbiota structure and abundance between the post-cholecystectomy non-diarrhea (PCND) and post-cholecystectomy diarrhea (PCD) groups. Linear discriminant analysis and effect size analysis show the taxa with the greatest differences in abundance between the PCND and PCD groups. Yellow bars: PCND group-enriched taxa; Green bars: PCD group-enriched taxa.
Abundance in Group 1: increased abundance in post-cholecystectomy diarrhea (PCD)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidales | ||
| Bacteroidia | ||
| Betaproteobacteria | ||
| Burkholderiales | ||
| Prevotella | ||
| Prevotellaceae | ||
| Sutterella |
Revision editor(s): Chinelsy, MyleeeA, ChiomaBlessing
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