Ambient temperature alters body size and gut microbiota of Xenopus tropicalis

Study information

Reviewed Marked as Reviewed by Folakunmi on 2024-1-14

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31686318

DOI Digital object identifier for electronic documents.

10.1007/s11427-019-9540-y

URI

Authors

Li J, Rui J, Li Y, Tang N, Zhan S, Jiang J, Li X

Journal

Science China. Life sciences

Year

2020

Keywords:

Xenopus tropicalis, body size, gut microbiota, temperature, thermal adaptation

Temperature is important to determine physiological status of ectotherms. However, it is still not fully understood how amphibians and their symbiotic microbiota acclimate to ambient temperature. In this study, we investigated the changes of gut microbiota of Xenopus tropicalis at different temperatures under controlled laboratory conditions. The results showed that microbial communities were distinct and shared only a small overlap among froglet guts, culture water and food samples. Furthermore, the dominant taxa harbored in the gut exhibited low relative abundance in water and food. It indicates that bacterial taxa selected by amphibian gut were generally of low abundance in the external environment. Temperature could affect beta-diversity of gut microbiota in terms of phylogenetic distance, but it did not affect alpha diversity. The composition of gut microbiota was similar in warm and cool treatments. However, signature taxa in different temperature environments were identified. The relationships between temperature, gut microbiota and morphology traits of X. tropicalis revealed in this study help us to predict the consequences of environmental changes on ectothermic animals.

Experiment 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-14

Curated date: 2023/10/12

Curator: Chikamso

Revision editor(s): Chikamso, Folakunmi

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Xenopus tropicalis

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Midgut Mesenteron,Midgut,midgut

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Temperature Temperature,temperature

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Cool Gut

Group 1 name Corresponds to the case (exposed) group for case-control studies: Warm Gut

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Xenopus tropicalis gut microbiota response to warm environmental conditions

Group 0 sample size Number of subjects in the control (unexposed) group: 23

Group 1 sample size Number of subjects in the case (exposed) group: 28

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): None

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: DESeq2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-14

Curated date: 2023/10/12

Curator: Chikamso

Revision editor(s): Chikamso, Peace Sandy, Folakunmi

Source: Table S4 (Genus level)

Description: Different abundance of gut microbiota between warm (group 1) and cool (group 0) environment (Warm Gut vs. Cool Gut)

Abundance in Group 1: increased abundance in Warm Gut

NCBI	Quality Control	Links
Akkermansia
Anaerotruncus
Coprobacillus
Parabacteroides
Thalassospira

Revision editor(s): Chikamso, Peace Sandy, Folakunmi

Signature 2

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-14

Curated date: 2023/10/12

Curator: Chikamso

Revision editor(s): Peace Sandy, Folakunmi, Chikamso, OdigiriGreat, Chinelsy

Source: Table S4 (Genus level)

Description: Different abundance of gut microbiota between warm (group 1) and cool (group 0) environment (Warm Gut vs. Cool Gut)

Abundance in Group 1: decreased abundance in Warm Gut

NCBI	Quality Control	Links
Citrobacter
Anoxybacillus
Escherichia/Shigella sp.
Anaerosporobacter
Romboutsia
Acinetobacter
Janthinobacterium

Revision editor(s): Peace Sandy, Folakunmi, Chikamso, OdigiriGreat, Chinelsy