Ambient temperature alters body size and gut microbiota of Xenopus tropicalis

Study information

Needs review

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

31686318

DOI Digital object identifier for electronic documents.

10.1007/s11427-019-9540-y

URI

Authors

Li J, Rui J, Li Y, Tang N, Zhan S, Jiang J, Li X

Journal

Science China. Life sciences

Year

2020

Keywords:

Xenopus tropicalis, body size, gut microbiota, temperature, thermal adaptation

Temperature is important to determine physiological status of ectotherms. However, it is still not fully understood how amphibians and their symbiotic microbiota acclimate to ambient temperature. In this study, we investigated the changes of gut microbiota of Xenopus tropicalis at different temperatures under controlled laboratory conditions. The results showed that microbial communities were distinct and shared only a small overlap among froglet guts, culture water and food samples. Furthermore, the dominant taxa harbored in the gut exhibited low relative abundance in water and food. It indicates that bacterial taxa selected by amphibian gut were generally of low abundance in the external environment. Temperature could affect beta-diversity of gut microbiota in terms of phylogenetic distance, but it did not affect alpha diversity. The composition of gut microbiota was similar in warm and cool treatments. However, signature taxa in different temperature environments were identified. The relationships between temperature, gut microbiota and morphology traits of X. tropicalis revealed in this study help us to predict the consequences of environmental changes on ectothermic animals.

Experiment 1

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Curated date: 2023/10/12

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Xenopus tropicalis

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Midgut Mesenteron,Midgut

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: temperature temperature

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Cool Temperature Treatment

Group 1 name Corresponds to the case (exposed) group for case-control studies: Warm Temperature Treatment

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Xenopus tropicalis gut microbiota response to warm environmental conditions

Group 0 sample size Number of subjects in the control (unexposed) group: 23

Group 1 sample size Number of subjects in the case (exposed) group: 28

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): None

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.1

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 0

Matched on Factors on which subjects have been matched on in a case-control study: Matched on: "water" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.water, Matched on: "gut" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.gut, Matched on: "food" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.food

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: Confounders controlled for: "temperature" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.temperature, Confounders controlled for: "water quality" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.water quality, diet, Confounders controlled for: "humidity" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.humidity, Confounders controlled for: "aeration" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.aeration, Confounders controlled for: "light/dark cycle" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.light/dark cycle

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2023/10/12

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Source: Table S4 (Phylum level)

Description: Different abundance of gut microbiota between warm (group 1) and cool (group 0) environment (Warm Gut vs. Cool Gut)

Abundance in Group 1: decreased abundance in Warm Temperature Treatment

NCBI	Quality Control	Links
Armatimonadota
Bacillota
Chlamydiales
Mycoplasmatota
Pseudomonadota

Revision editor(s): Chikamso, Peace Sandy

Signature 2

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Curator: Chikamso

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Source: Table S4 (Phylum level)

Description: Different abundance of gut microbiota between warm (group 1) and cool (group 0) environment (Warm Gut vs. Cool Gut)

Abundance in Group 1: increased abundance in Warm Temperature Treatment

NCBI	Quality Control	Links
Acidobacteriota
Actinomycetota
Aquificae
Bacteroidota
Candidatus Absconditabacteria
Candidatus Dependentiae bacterium
Candidatus Dojkabacteria
Candidatus Gracilibacteria
Candidatus Marinimicrobia
Candidatus Parcubacteria
Candidatus Saccharibacteria
Chlorobiota
Chloroflexia
Cyanobacteriota
Deinococcota
Fusobacteriota
Planctomycetia
Synergistota
Verrucomicrobiota
Terrabacteria group bacterium

Revision editor(s): Chikamso, OdigiriGreat, Chinelsy, Peace Sandy