Modified Mediterranean-ketogenic diet modulates gut microbiome and short-chain fatty acids in association with Alzheimer's disease markers in subjects with mild cognitive impairment
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Cognitive impairment Abnormality of cognition,Cognitive abnormality,Cognitive defects,Cognitive deficits,Cognitive impairment,Intellectual impairment,cognitive impairment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Cognitive Normal
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mild Cognitive Impairment
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants diagnosed with early Mild Cognitive Impairment(diagnosed using ADNI-2 criteria)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 11
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Fig. 1. and Result Text
Description: Differences in the gut microbiome between subjects clinically diagnosed with mild cognitive impairment (MCI) versus cognitively normal (CN) counterparts.
Abundance in Group 1: increased abundance in Mild Cognitive Impairment
NCBI | Quality Control | Links |
---|---|---|
Coprococcus | ||
Coriobacteriaceae | ||
Enterobacteriaceae | ||
Phascolarctobacterium | ||
Pseudomonadota | ||
MogibacteriaceaeMogibacteriaceae | ||
Mycoplasmatota | ||
Bacillota |
Signature 2
Source: Fig. 1. and Result Text
Description: Differences in the gut microbiome between subjects clinically diagnosed with mild cognitive impairment (MCI) versus cognitively normal (CN) counterparts.
Abundance in Group 1: decreased abundance in Mild Cognitive Impairment
NCBI | Quality Control | Links |
---|---|---|
Dialister |
Revision editor(s): MyleeeA
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Post AHAD in MCI
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Post MMKD in MCI
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants diagnosed with early Mild Cognitive impairment who were given the diet Intervention Modified Mediterrean Ketogenic Diet (MMKD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 11
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Fig 4 and Text
Description: Mediterranean-style ketogenic diet (MMKD) and American Heart Association Diet (AHAD) induce specific changes in the gut microbiome of subjects clinically diagnosed with mild cognitive impairment (MCI) versus cognitively normal (CN) counterparts.
Abundance in Group 1: decreased abundance in Post MMKD in MCI
NCBI | Quality Control | Links |
---|---|---|
Bifidobacteriaceae | ||
Bifidobacterium |
Revision editor(s): MyleeeA
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Post AHAD in CN and MCI
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Post MMKD in CN and MCI
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- All Participants diagnosed with early Mild Cognitive impairment and diagnosed as Cognitively Normal who were given the diet Intervention Modified Mediterranean Ketogenic Diet (MMKD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 17
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Fig 4
Description: Mediterranean-style ketogenic diet (MMKD) and American Heart Association Diet (AHAD) induce specific changes in the gut microbiome of subjects clinically diagnosed with mild cognitive impairment (MCI) versus cognitively normal (CN) counterparts.
Abundance in Group 1: decreased abundance in Post MMKD in CN and MCI
NCBI | Quality Control | Links |
---|---|---|
Bifidobacteriaceae | ||
Bifidobacterium |
Revision editor(s): MyleeeA
Experiment 4
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Pre MMKD in CN and MCI
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants diagnosed as Cognitively Normal and diagnosed with early Mild Cognitive impairment who were given the diet Intervention Modified Mediterrean Ketogenic Diet (MMKD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 11
- Group 1 sample size Number of subjects in the case (exposed) group
- 11
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Supplemental Figure 4
Description: Lefse analysis showing bacterial taxa contributing to the microbiome difference between the two diet groups.
Abundance in Group 1: decreased abundance in Post MMKD in CN and MCI
NCBI | Quality Control | Links |
---|---|---|
Actinomycetota | ||
Bifidobacteriaceae | ||
Bifidobacteriales | ||
Bifidobacterium | ||
Lactococcus |
Revision editor(s): MyleeeA