Effect of gut microbiome modulation on muscle function and cognition: the PROMOTe randomised controlled trial/Experiment 4
From BugSigDB
Reviewed Marked as Reviewed by Chloe on 2024-11-22
Subjects
- Location of subjects
- United Kingdom
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet Dietary,Diets,Diet,diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Baseline Prebiotic
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- End of study Prebiotic
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Week 12 (End of study) participants who received daily prebiotic supplementation (inulin and fructo-oligosaccharides) along with branched-chain amino acids (BCAA) and resistance exercise.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 36
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months.
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- Linear Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- Matched on: "twin pairs" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.twin pairs
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex, Confounders controlled for: "zygosity" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.zygosity, Confounders controlled for: "baseline microbiota" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.baseline microbiota
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by Chloe on 2024-11-22
Curated date: 2024/10/11
Curator: Rukaya-lab
Revision editor(s): MyleeeA, Rahila, Chrisawoke, Rukaya-lab
Source: Supplementary Figure S6
Description: Significant Microbiota Features identified between Baseline Prebiotic and Study End Prebiotic using Linear models.
Abundance in Group 1: increased abundance in End of study Prebiotic
Revision editor(s): MyleeeA, Rahila, Chrisawoke, Rukaya-lab
Signature 2
Reviewed Marked as Reviewed by Chloe on 2024-11-22
Curated date: 2024/10/11
Curator: Rukaya-lab
Revision editor(s): MyleeeA, Rahila, Chrisawoke, Rukaya-lab
Source: Supplementary Figure S6
Description: Significant Microbiota Features identified between Baseline Prebiotic and Study End Prebiotic using Linear models.
Abundance in Group 1: decreased abundance in End of study Prebiotic
Revision editor(s): MyleeeA, Rahila, Chrisawoke, Rukaya-lab
